Paper Title :Improvement Of In Vitro Developmental Competence Of OOCYTES By Short-Term Exposure To Mild Stress Induced By Hydrogen Peroxide During OOCYTE Maturation
Author :Kavan Nikdel, Mehdi Aminafshar, Nasser Emamjomeh-Kashan, Mahboobeh Khajenabi, Abdollah Mohammadi-Sangcheshmeh
Article Citation :Kavan Nikdel ,Mehdi Aminafshar ,Nasser Emamjomeh-Kashan ,Mahboobeh Khajenabi ,Abdollah Mohammadi-Sangcheshmeh ,
(2016 ) " Improvement Of In Vitro Developmental Competence Of OOCYTES By Short-Term Exposure To Mild Stress Induced By Hydrogen Peroxide During OOCYTE Maturation " ,
International Journal of Advances in Science, Engineering and Technology(IJASEAT) ,
pp. 16-18,
Volume-4,Issue-2
Abstract : In this study the effects of hydrogen peroxide (H2O2) on developmental competence of oocytes were evaluated in
ovine model. In vitro maturation was performed in presence of various concentrations (0, 10, 100, or 1000 μM) of H2O2.
Levels of intracellular glutathione (GSH), embryonic cleavage, and blastocyst formation following in vitro fertilization (IVF)
were determined. When oocytes treated with 10 μM H2O2, a higher GSH level was observed in comparison with the control
group, however oocytes exposed to 1000 μM H2O2 indicated the lowest GSH level. A higher rate of blastocyst formation was
also observed in 100 μM H2O2 group as compared with the control group. Our results show that H2O2 treatment during IVM
had a beneficial effect on embryonic development in vitro.
Index Terms— Blastocyst, GSH level, Hydrogen peroxide (H2O2), Oocyte
Type : Research paper
Published : Volume-4,Issue-2
DOIONLINE NO - IJASEAT-IRAJ-DOIONLINE-4462
View Here
Copyright: © Institute of Research and Journals
|
|
| |
|
PDF |
| |
Viewed - 58 |
| |
Published on 2016-05-16 |
|