Paper Title :The Effect of Bovine Serum Albumin to Platform of Electrochemical Immunosensor for Hepatocellular Carcinoma Detection
Author :Thipnantha Pitukdechatanakit, Porntip Khownarumit, Dujduan Waraho-Zhmayev, Rungtiva P. Poo-Arporn
Article Citation :Thipnantha Pitukdechatanakit ,Porntip Khownarumit ,Dujduan Waraho-Zhmayev ,Rungtiva P. Poo-Arporn ,
(2017 ) " The Effect of Bovine Serum Albumin to Platform of Electrochemical Immunosensor for Hepatocellular Carcinoma Detection " ,
International Journal of Advances in Science, Engineering and Technology(IJASEAT) ,
pp. 63-67,
Volume-5, Issue-4, Spl. Iss-2
Abstract : Hepatocellular carcinoma (HCC) diagnosis is commonly accomplished by biomarker testing. Alpha-fetoprotein
(AFP) is the most widely used biomarker for HCC during the past several decades. Most of the biomarker tests that have
been developed are time-consuming and not sensitive enough for HCC early screening. To overcome these limitations, an
alternative method for AFP detection based on electrochemical immunosensor was developed using Electrochemical
Impedance Spectroscopy (EIS) technique. An immunoassay based on direct Enzyme-Linked Immunosorbent Assay (ELISA)
was performed on a commercial screen-printing carbon electrode (SPCE) instead of a 96-well plate. 1-pyrenebutanoic acid
succinimidyl ester was used as an immobilizing agent to immobilize AFP antibody effectively on the electrode surface.
However, this platform needed to be adjusted for signal enhancement because AFP antigen-antibody complex molecules on
SPCE was so small that cannot bring about significant electrical signal change. Thereby, many different amounts of Bovine
Serum Albumin (BSA) was optimized for signal enhancement purpose. The results showed that mixing 0.2 % wt/vol BSA
with AFP can enhance signal of the specific antigen-antibody complex molecules considerably as it created more double
layer charges between electrode surface and Fe(CN)6
3-/4-. More double layer charges hindered Fe(CN)6
3-/4- to diffuse from
bulk solution to electrode surface and caused more resistance at the interface. On the other hand, mixing 0.5% and 1% wt/vol
BSA with AFP concealed the signal of AFP antigen-antibody complex molecules. Too much amount of BSA created too
much double layer charges that overcame small impedance signal change from the formation of AFP antigen-antibody
complex molecules.
Keywords: Hepatocellular Carcinoma (HCC), Alpha-Fetoprotein (AFP), Electrochemical Impedance Spectroscopy (EIS),
Direct Enzyme-Linked Immunosorbent Assay (ELISA), Screen-Printing Carbon Electrode (SPCE), 1-Pyrenebutanoic Acid
Succinimidyl Ester, Bovine Serum Albumin (BSA)
Type : Research paper
Published : Volume-5, Issue-4, Spl. Iss-2
DOIONLINE NO - IJASEAT-IRAJ-DOIONLINE-10555
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Copyright: © Institute of Research and Journals
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Published on 2018-02-13 |
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