Paper Title
In Vitro Behavior of Zn-Modified Calcium Phosphate Fine Powders
Abstract
Zinc modified (Zn/(Zn+Ca) = 0, 0.03, 0.13) calcium phosphate fine powders were synthesized by biomimetic
approach and subsequent high-temperature treatment. Bi-phase mixture of β-tricalcium phosphate (β-TCP) and
hydroxyapatite (HA) was prepared at non modified sample and monophase Zn-β-TCP at Zn modified samples. The in vitro
biological behavior of the prepared powders was studied using human Lep-3 and bovine MDBK cells. The effect of the
materials on cell viability and proliferation was evaluated by three methods with different molecular / cellular targets and
mechanisms of action - MTT test, neutral red uptake assay and crystal violet staining. The results obtained revealed high
viability of both Lep-3 and MDBK cells cultured for 72h and 96h in the presence of the materials examined applied as 100%
material-modified cell culture medium in all three cases. The dilution of material-modified cell culture medium with nonmodified
culture medium leads to increasing of cell viability.